CD28 Protein (C-human IgG1-Fc, Human)
Product Specifications
Abbreviation
CD28
Synonyms
Tp44
Host
HEK 293
Tag
C-human IgG1-Fc
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .
Bioactivity
1.Loaded Recombinant Human B7-1 Protein, His Tag (Cat. No. 10698-H08H) on His1K Biosensor, can bind Recombinant Human CD28 Protein, hFc Tag (Cat. No. 11524-H02H) with an affinity constant of 0.46 μM as determined in BLI assay (Sartorius Octet RED384) (QC tested) . 2.Loaded Recombinant Human CD86 Protein, His Tag (Cat. No. 10699-H08H) on His1K Biosensor, can bind Recombinant Human CD28 Protein, hFc Tag (Cat. No. 11524-H02H) with an affinity constant of 2.72 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .
Function
Met1-Pro152
Molecular Weight
The recombinant human CD28/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 375 amino acids and predictes a molecular mass of 42 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCD28/Fc monomer is approximately 55-65 kDa due to glycosylation. The molecular weight of this protein is around 112.6 kDa verified by SEC-MALS (Routinely tested) .
Species
Human
Curated Selection
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