CD28 Protein (C-human IgG1-Fc, Cynomolgus)
Product Specifications
Abbreviation
CD28
Host
HEK 293
Tag
C-human IgG1-Fc
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
> 90 % as determined by SDS-PAGE.
Bioactivity
1. Immobilized Recombinant Human CD86 / B7-2 Protein (His Tag) (Cat:10699-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus CD28 / TP44 Protein (Fc Tag, ECD) (Cat:90182-C02H), the EC50 is 100-420 ng/mL. 2. Immobilized Recombinant Human CD80 / B7-1 Protein (His Tag) (Cat:10698-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus CD28 / TP44 Protein (Fc Tag, ECD) (Cat:90182-C02H), the EC50 is 600-1400 ng/mL. 3.Loaded Recombinant Cynomolgus CD28 Protein, hFc Tag (Cat. No. 90182-C02H) on proA Biosensor, can bind Recombinant Human B7-1/CD80 Protein, His Tag (Cat. No. 10698-H08H) with an affinity constant of 0.126 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Loaded Recombinant Cynomolgus CD28 Protein, hFc Tag (Cat. No. 90182-C02H) on proA Biosensor, can bind Recombinant Human B7-2/CD86 Protein, His Tag (Cat. No. 10699-H08H) with an affinity constant of 0.359 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .
Function
Met1-Pro152
Molecular Weight
The recombinant cynomolgus CD28 consists 372 amino acids and predicts a molecular mass of 41.9 kDa.
Species
Cynomolgus
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