CD28 Protein (C-His, Human, Cynomolgus, Rhesus)
Product Specifications
Abbreviation
CD28
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS (Routinely tested) .
Bioactivity
1.Immobilized Recombinant Human / Cynomolgus / Rhesus CD28 Protein (ECD, His Tag) (Cat: 90182-C08H) at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus / Rhesus CD80 / B7-1 Protein (Fc Tag) (Cat: 90268-C02H), the EC50 is 150-600 ng/mL. 2.Loaded Recombinant Human B7-1/CD80 Protein, hFc Tag (Cat. No. 10698-H02H) on proA Biosensor, can bind Recombinant Human, Cynomolgus, Rhesus CD28 Protein, His Tag (Cat. No. 90182-C08H) with an affinity constant of 0.895 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .
Function
Asn19-Pro152
Molecular Weight
The recombinant human/cynomolgus/rhesus CD28 consists of 145 amino acids and predicts a molecular mass of 16.6 kDa. The molecular weight of this protein is around 64.5 kDa verified by SEC-MALS (Routinely tested) .
Species
Human, Cynomolgus, Rhesus
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