CD274 Protein (C-mouse IgG2a-Fc, Human)

Product Specifications

Abbreviation

CD274

Synonyms

B7-H, B7-H1, B7H1, hPD-L1, PD-L1, PDCD1L1, PDCD1LG1, PDL1

Host

HEK 293

Tag

C-mouse IgG2a-Fc

Endotoxin

< 0.05 EU per mg protein.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Recombinant Human PD-L1 / B7-H1 / CD274 Protein (mFc Tag) (Cat: 10084-H38H-UE) at 2 μg/mL (100 μL/well) can bind Recombinant Human PD1 / PDCD1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10377-H41H-B), the EC50 is 8-25 ng/mL (Routinely tested) . 2.Loaded Recombinant Human PD-L1 / B7-H1 / CD274 Protein (mFc Tag) (Cat. No. 10084-H38H-UE) on ProA Biosensor, can bind Recombinant Human PD1 / PDCD1 Protein (His Tag) (Cat. No. 10377-H08H) with an affinity constant of 0.17 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-Thr239

Molecular Weight

The recombinant mature human B7-H1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 459 amino acids and predicts a molecular mass of 52 kDa. As a result of glycosylation, the human B7-H1/Fc monomer migrates as an approximately 65-70 kDa protein in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 121.6 kDa verified by SEC-MALS (Routinely tested) .