CD274 Protein (C-human IgG1-Fc & AVI, Human)

1.Immobilized Recombinant Human PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat: 10377-H02H) at 2 μg/mL (100 μl/well) can bind Recombinant Human PD-L1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10084-H41H-B), the EC50 is 25-75 ng/mL (QC tested) . 2.Serial dilutions of Nivolumab derived Anti-PD1 Antibody were added into Recombinant Human PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat: 10377-H02H) : Recombinant Human PD-L1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10084-H41H-B) binding reactions. The half maximal inhibitory concentration (IC50) is 1.7 μg/mL (Routinely tested) . 3.Serial dilutions of Anti-PD1 (Research grade Nivolumab biosimilar) were added into Recombinant Human PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat: 10377-H02H) : Recombinant Human PD-L1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10084-H41H-B) binding reactions. The half maximal inhibitory concentration (IC50) is 1.7 μg/mL (Routinely tested) .

The recombinant Human CD274 consisting of 474 amino acids and has a calculated molecular mass of 53.83 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 71.4 kDa protein in SDS-PAGE under reducing conditions.