CD274 Protein (C-human IgG1-Fc & AVI, Cynomolgus, Rhesus)

1.Immobilized Recombinant Cynomolgus PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat: 90311-C02H) at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus / Rhesus PD-L1/B7-H1 Protein (Fc & AVI Tag), Biotinylated (Cat: 90251-C41H-B), the EC50 is 12-36 ng/mL (QC tested) . 2.Biotinylated Recombinant Cynomolgus, Rhesus PD-L1/B7-H1 Protein, hFc & AVI Tag (Cat. No. 90251-C41H-B) captured on Protein A chip, can bind Recombinant Cynomolgus PD-1 Protein, His Tag (Cat. No. 90311-C08H) with an affinity constant of 1.200 μM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 3.Immobilized Anti-PDL1 (Research grade Atezolizumab biosimilar) at 5 μg/mL (100 μL/well) can bind Recombinant Cynomolgus / Rhesus PD-L1/B7-H1 Protein (Fc & AVI Tag), Biotinylated (Cat: 90251-C41H-B), the EC50 is 1.2-3.6 ng/mL (Routinely tested) . 4.Immobilized Recombinant Cynomolgus, Rhesus PD-L1/B7-H1 Protein (hFc & AVI Tag), Biotinylated, HPLC-verified (Cat: 90251-C41H-B) at 5 μg/mL (100 μl/well) on streptavidin precoated (5 μg/mL, 100 μL/well) can bind Recombinant Cynomolgus PD-1 Protein (His Tag), HPLC-verified (Cat: 90311-C08H), the EC50 is 187.75-563.55 ng/mL (Routinely tested) .

The recombinant Cynomolgus / Rhesus CD274 consisting of 474 amino acids and has a calculated molecular mass of 53.83 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 69.1 kDa protein in SDS-PAGE under reducing conditions.