CD274 Protein (C-His-AVI, Human)

1.Immobilized Recombinant Human PD-L1 Protein (His & AVI Tag), Biotinylated (Cat: 10084-H49H-B) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat: 10377-H02H), the EC50 is 3-10 ng/mL (QC tested) . 2.Immobilized Anti-PDL1 Antibody (Atezolizumab), Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human PD-L1 Protein (His & AVI Tag), Biotinylated (Cat: 10084-H49H-B), the EC50 is 2-6 ng/mL (Rountinely tested) . 3.Immobilized Recombinant Human PD-L1 Protein (His & AVI Tag), Biotinylated (Cat: 10084-H49H-B) at 1 μg/mL (100 μL/well) on streptavidin precoated (5 μg/mL, 100 μL/well) plate can bind Anti-PDL1 (Research grade Atezolizumab biosimilar), the EC50 is 1-4 ng/mL (Routinely tested) . 4.Immobilized Recombinant Human PD-L1 Protein (His & AVI Tag), Biotinylated (Cat: 10084-H49H-B) at 1 μg/mL (100 μL/well) on streptavidin precoated (5 μg/mL, 100 μL/well) plate can bind Recombinant Human PD1 / PDCD1 / CD279 Protein (ECD, Fc Tag) (Cat: 10377-H05H), the EC50 is 3.3 ng/mL (Routinely tested) .

The recombinant human CD274 consists of 246 amino acids and predicts a molecular mass of 28.48 kDa. It migrates as an approximately 38.01 kDa band in SDS-PAGE under reducing conditions.