CD274 Protein (C-human IgG1-Fc, Rat)

Product Specifications

Abbreviation

CD274

Synonyms

Pdcd1lg1, RGD1566211

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized rat CD274-Fc at 10 μg/ml (100 μl/well) can bind biotinylated rat PDCD1-Fc (Cat:80448-R02H), The EC50 of biotinylated rat PDCD1-Fc (Cat:80448-R02H) is 0.14-0.34 μg/ml.

Function

Met1-Thr238

Molecular Weight

The recombinant rat CD274/Fc is a disulfide-linked homodimer. The reduced monomer comprises 459 amino acids and has a predicted molecular mass of 51.8 kDa. The apparent molecular mass of the protein is approximately 73.2 kDa in SDS-PAGE under reducing conditions.