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CD274 Protein (C-His-AVI, Mouse)

Product Specifications

Abbreviation

CD274

Synonyms

A530045L16Rik, B7h1, Cd274, Pdcd1l1, Pdcd1lg1, Pdl1

Host

HEK 293

Tag

C-His-AVI

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Anti-PDL1 (GEN) -IgG1 Antibody (Atezolizumab) (Cat: 68049-H001) at 2 μg/mL (100 μL/well) can bind Recombinant Mouse PD-L1 Protein (His & AVI Tag), Biotinylated (Cat: 50010-M49H-B), the EC50 is 0.3-1.0 ng/mL (QC tested) . 2.Immobilized Recombinant Mouse PD-L1/B7-H1 Protein (His & AVI Tag), Biotinylated, HPLC-verified (Cat: 50010-M49H-B) at 1 μg/mL (100 μl/well) on streptavidin precoated (5 μg/mL, 100 μL/well) can bind Recombinant Mouse PD-1 Protein (ECD, hFc Tag), HPLC-verified (Cat: 50124-M02H), the EC50 is 61.95-185.85 ng/mL (Routinely tested) .

Function

Met1-Thr238

Molecular Weight

The recombinant Mouse CD274 consists of 246 amino acids and predicts a molecular mass of 28.02 kDa. It migrates as an approximately 46.7 kDa band in SDS-PAGE under reducing conditions.

Species

Mouse

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