CD274 Protein (C-human IgG1-Fc, Feline)

Product Specifications

Abbreviation

CD274

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Loaded Biotinylated Recombinant Feline PD-1 Protein, His & AVI Tag (Cat. No. 62013-U49H-B) on HIS1K Biosensor, can bind Recombinant Feline PD-L1/B7-H1 Protein, Fc Tag (Cat. No. 62014-U02H) with an affinity constant of 69.700 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-His239

Molecular Weight

The recombinant Feline CD274 comprises 459 amino acids and has a predicted molecular mass of 51.82 kDa. It migrates as approximately 61.3 kDa band in SDS-PAGE under reducing conditions.