CD274 Protein (Native, Human)

1.Immobilized Recombinant Human PD-L1 / B7-H1 / CD274 Protein (Cat: 10084-HNAH) at 2 μg/mL (100 μL/well) can bind Anti-PD-L1 Antibody, Human IgG1 (Atezolizumab), the EC50 is 4-15 ng/mL. (QC tested) . 2.Anti-PDL1 Antibody, Human IgG1 captured on Protein A chip can bind Recombinant Human PD-L1/B7-H1 Protein (Cat. No. 10084-HNAH) with an affinity constant of 8.81 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 3.Loaded Recombinant Human PD-1 Protein, hFc Tag (Cat. No. 10377-H02H) on AHC Biosensor, can bind Recombinant Human PD-L1/B7-H1 Protein (Cat. No. 10084-HNAH) with an affinity constant of 2.800 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human CD274 consists of 220 amino acids and predicts a molecular mass of 25.2 kDa. As a result of glycosylation, the human CD274 migrates as an approximately 36 kDa protein in SDS-PAGE under reducing conditions.