CD274 Protein (C-His, Mouse)

1.Loaded Recombinant Mouse PD-L1 / B7-H1 / CD274 Protein (ECD, His Tag) (Cat. No. 50010-M08H-UE) on His1K Biosensor, can bind Recombinant Mouse PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat. No. 50124-M02H) with an affinity constant of 0.388 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 2.Recombinant Mouse PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat: 50124-M02H) captured on Protein A chip, can bind Recombinant Mouse PD-L1 / B7-H1 / CD274 Protein (ECD, His Tag) (Cat: 50010-M08H-UE) with an affinity constant of 2.273 μM as determined in an SPR assay (Biacore T200) (Routinely tested) .

The secreted recombinant mouse CD274 comprises 231 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, the band migrates approximately 45-55 kDa in the SDS-PAGE under reducing conditions. The molecular weight of this protein is around 37.1 kDa verified by SEC-MALS (Routinely tested) .