CD27 Protein (C-human IgG1-Fc & His, Mouse)

Product Specifications

Abbreviation

CD27

Synonyms

Cd27, S152, Tnfrsf7, Tp55

Host

HEK 293

Tag

C-human IgG1-Fc & His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 94 % as determined by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA . Immobilized recombinant mouse CD27 at 2 μg/ml (100 μl/well) can bind biotinylated human CD70 with a linear range of 0.39-12.5 ng/ml .

Function

Met1-Arg182

Molecular Weight

The recombinant mouse CD27/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 410 amino acids and has a predicted molecular mass of 46.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD27/Fc monomer is approximately 60-65 kDa due to glycosylation.