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CD22 Protein (C-His-AVI, Human)

Product Specifications

Abbreviation

CD22

Synonyms

SIGLEC-2, SIGLEC2

Host

HEK 293

Tag

C-His-AVI

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Anti-CD22 Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human Siglec-2/CD22 Protein (ECD, His & AVI Tag), Biotinylated (Cat: 11958-H49H-B), the EC50 is 4-12 ng/mL (QC tested) . 2.Loaded anti-human CD22 antibody, human IgG1 on AHC2 Biosensor, can bind Biotinylated Recombinant Human Siglec-2/CD22 Protein, ECD, His & AVI Tag (Cat. No. 11958-H49H-B) with an affinity constant of 1.570 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-Arg687

Molecular Weight

The recombinant Human CD22 consists of 694 amino acids and predicts a molecular mass of 78.41 kDa. It migrates as an approximately 111.4 kDa band in SDS-PAGE under reducing conditions.