CD2 Protein (C-His, Human)

Product Specifications

Abbreviation

CD2

Synonyms

LFA-2, SRBC, T11

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 97 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Measured by its binding ability in a functional ELISA. Immobilized Human CD2 His (Cat:10982-H08H) at 2 μg/ml (100 μl/well) can bind Human CD58 hFc (Cat:12409-H02H), the EC50 of Human CD58 hFc is 7.0-28.0 ng/mL. 2.Loaded Recombinant Human CD58/LFA-3 Protein, hFc Tag (Cat. No. 12409-H02H) on ProA Biosensor, can bind Recombinant Human CD2 Protein, His Tag (Cat. No. 10982-H08H) with an affinity constant of 1.36 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-Asp209

Molecular Weight

The recombinant human CD2 consists of 196 amino acids and has a predicted molecular mass of 22.7 kDa. In SDS-PAGE under reducing conditions, rhCD2 magrates as an approximately 37 kDa band due to glycosylation.