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CD19 Protein (C-His-AVI, Human)

Product Specifications

Abbreviation

CD19

Synonyms

B4, CVID3

Host

HEK 293

Tag

C-His-AVI

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE.

Bioactivity

1.Immobilized Anti-CD19 (Research Grade Inebilizumab Biosimilar) at 2μg/mL (100μL/well) can bind Recombinant Human CD19 Protein (ECD, His & AVI Tag), Biotinylated (Cat: 11880-H49H-B), the EC50 is 3-9 ng/mL (QC tested) . 2.Anti-CD19 (Research Grade Inebilizumab Biosimilar) captured on Protein A chip can bind Biotinylated Recombinant Human CD19 Protein, His & AVI Tag (Cat. No. 11880-H49H-B) with an affinity constant of 1.234 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3.Anti-CD19 (Research Grade Tafasitamab Biosimilar) captured on Protein A chip can bind Biotinylated Recombinant Human CD19 Protein, His & AVI Tag (Cat. No. 11880-H49H-B) with an affinity constant of 17.630 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 4.Anti-CD19 (Research Grade Coltuximab Biosimilar) captured on Protein A chip can bind Biotinylated Recombinant Human CD19 Protein, His & AVI Tag (Cat. No. 11880-H49H-B) with an affinity constant of 5.155 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 5.Recombinant Human CD19 Protein (ECD, His & AVI Tag), Biotinylated (Cat: 11880-H49H-B) captured on Biotin CAP-Series S Sensor chip, can bind Anti-CD19 (Research Grade Inebilizumab Biosimilar) with an affinity constant of 94.700 pM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

Function

Met1-Lys291

Molecular Weight

The recombinant Human CD19 consists of 298 amino acids and predicts a molecular mass of 33.37 kDa. It migrates as an approximately 56.84 kDa band in SDS-PAGE under reducing conditions.

Species

Human

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