CCR2 Protein (N-Flag & C-His, Human)

Product Specifications

Abbreviation

CCR2

Synonyms

CC-CKR-2, CCR-2, CCR2A, CCR2B, CD192, CKR2, CKR2A, CKR2B, CMKBR2, FLJ78302, MCP-1-R, PCLUD

Host

HEK 293

Tag

N-Flag & C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 90 % as determined by SDS-PAGE.

Bioactivity

1.Immobilized recombinant Human CCR2b Protein (Cat: 16084-H18H-NA) at 5 μg/mL (100 μL/well) can bind Plozalizumab-Derived anti-CCR2 Antibody, Human IgG1, the EC50 is 9-27 ng/mL. 2.Loaded Plozalizumab-Derived anti-CCR2 Antibody, Human IgG1 on ProA Biosensor, can bind Human CCR2-Nanodisc Protein, His & Flag Tag (Cat. No. 16084-H18H-NA) with an affinity constant of 29.9 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Leu2-Arg231 & Glu235-Leu360

Molecular Weight

The recombinant human CCR2b predicts a molecular mass of 49.12 kDa. The apparent molecular mass of the protein is approximately 41 kDa in SDS-PAGE under reducing conditions.