CA9 Protein (C-human IgG1-Fc, Human)

1.Measured by its esterase activity. The specific activity is >50 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 1 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. 2.Immobilized Recombinant Human Carbonic Anhydrase IX / CA9 Protein (Fc Tag) (Cat: 10107-H02H) at 2 μg/mL (100 μL/well) can bind Anti-CA9 Antibody, Human IgG4 (Girentuximab), the EC50 is 5-18 ng/mL.

The recombinant human CA9/Fc is a disulfide-linked homodimer. The reduced monomer consists of 615 amino acids and predicts a molecular mass of 67.7 kDa. As a result of glycosylation, the apparent molecular mass of rh CA9/Fc monomer is approximately 80-90 kDa in SDS-PAGE under reducing conditions.