CA9 Protein (C-human IgG1-Fc & AVI, Human)

Product Specifications

Abbreviation

CA9

Synonyms

CAIX, G250, MN

Host

HEK 293

Tag

C-human IgG1-Fc & AVI

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Anti-CA9 (Research Grade Girentuximab biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human Carbonic Anhydrase IX Protein (ECD, Fc&AVI Tag), Biotinylated (Cat: 10107-H41H-B), the EC50 is 0.8-3 ng/mL (QC tested) . 2.Immobilized Recombinant Human Carbonic Anhydrase IX Protein (ECD, Fc&AVI Tag), Biotinylated (Cat: 10107-H41H-B) at 1 μg/mL (100 μL/well) on streptavidin precoated (5 μg/mL, 100 μL/well) plate can bind anti-CA9 (Research Grade Girentuximab biosimilar), the EC50 is 4.3 ng/mL (Routinely tested) .

Function

Met1-Asp414

Molecular Weight

The recombinant Human CA9 consists of 630 amino acids and predicts a molecular mass of 69.51 kDa. It migrates as an approximately 82.1 kDa band in SDS-PAGE under reducing conditions.

Species

Human