BTC Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

BTC

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 5-40 ng/mL.

Function

Met1-Tyr111

Molecular Weight

The recombinant human BTC/Fc is a disulfide-linked homodimer. The reduced monomer comprises 321 amino acids and has a predicted molecular mass of 36 kDa. The apparent molecular mass of the protein is approximately 43-46 kDa in SDS-PAGE under reducing conditions.