BTC Protein (C-human IgG1-Fc & His, Mouse)

Product Specifications

Abbreviation

BTC

Synonyms

Bcn, Btc

Host

HEK 293

Tag

C-human IgG1-Fc & His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-10 ng/mL.

Function

Met1-Gln118

Molecular Weight

The recombinant mouse BTC/Fc is a disulfide-linked homodimer. The reduced monomer consists of 335 amino acids and has a predicted molecular mass of 38 kDa. As a result of glycosylation, the apparent molecular mass of rm BTC/Fc monomer migrates with an apparent molecular mass of 50-55 kDa in SDS-PAGE under reducing conditions.