BACE1 Protein (C-His, Human)

Product Specifications

Abbreviation

BACE1

Synonyms

ASP2, BACE, HSPC104, KIAA1149

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK (Dpn) RR-NH2, R&D Systems, Catalog # ES004. The specific activity is >1.5 pmoles/min/μg.

Function

Met1-Thr457

Molecular Weight

The recombinant human BACE1 consists of 446 amino acids and has a calculated molecular mass of 49.9 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 60-65 kDa protein in SDS-PAGE under reducing conditions.