APP Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

APP

Synonyms

A4, AAA, ABETA, ABPP, AD1, alpha-sAPP, APPI, CTFgamma, CVAP, PN-II, PN2, preA4

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp) -NH2, (R&D Systems, Catalog # ES002) . The IC50 value is < 1.2 nM.

Function

Met1-Leu669

Molecular Weight

The recombinant human APP/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. The reduced monomer consists of 890 amino acids and predicts a molecular mass of 101 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rhAPP/Fc monomer is approximately 150-160 kDa due to the glycosylation.