ANGPT2 Protein (C-AVI & His, Human)

1.Immobilized Recombinant Human Tie2 / CD202b / TEK Protein (ECD, His Tag) (Cat:10700-H08H) at 2 μg/ml (100 μl/well) can bind Recombinant Human Angiopoietin-2 Protein (AVI & His Tag), Biotinylated (Cat:10691-H27H1-B), the EC50 is 300-900 ng/mL. 2.Immobilized Recombinant Human Angiopoietin-2 Protein (AVI & His Tag), Biotinylated (Cat: 10691-H27H1-B) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human TIE-2 Protein (Fc Tag) (Cat: 10700-H02H), the EC50 is 80-250 ng/mL (Routinely tested) . 3.Loaded Recombinant Human TIE-2 Protein (hFc Tag) (Cat. No. 10700-H02H) on proA Biosensor, can bind Biotinylated Recombinant Human Angiopoietin-2/ANGPT2 Protein (His & AVI Tag) (Cat. No. 10691-H27H1-B) with an affinity constant of 0.547 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human ANGPT2 consists of 248 amino acids and predicts a molecular mass of 28.72 kDa. It migrates as an approximately 30.74 kDa band in SDS-PAGE under reducing conditions.