ALPL Protein (C-His, Human)

Product Specifications

Abbreviation

ALPL

Synonyms

AP-TNAP, APTNAP, HOPS, HPPA, HPPC, HPPI, HPPO, TNALP, TNAP, TNS-ALP, TNSALP

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE.

Bioactivity

1.Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP) . The specific activity is > 50, 000 pmoles/min/μg. 2.Immobilized Alkaline Phosphatase / ALPL, Rabbit PAb (Cat: 10440-T16) at 2 μg/mL (100 μL/well) can bind Recombinant Human Alkaline Phosphatase / ALPL Protein (His Tag) (Cat: 10440-H08H), the EC50 is 5-16 ng/mL.

Function

Met1-Ser502

Molecular Weight

The recombinant human ALPL consists of 496 amino acids and predicts a molecular mass of 55 kDa. As a result of glycosylation, rhALPL migrates as approximately 65 kDa band in SDS-PAGE under reducing conditions.