ACVRL1 Protein (C-human IgG1-Fc & His, Mouse)

Product Specifications

Abbreviation

ACVRL1

Synonyms

Acvrl1, Acvrlk1, Alk-1, Alk1

Host

HEK 293

Tag

C-human IgG1-Fc & His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE

Bioactivity

Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. The ED50 for this effect is typically 3-15ng/mL in the presence of 2 ng/mL of recombinant human BMP9.

Function

Met1-Pro119

Molecular Weight

The secreted recombinant mouse ALK1/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer comprises 345 amino acids with a predicted molecular mass of 39 kDa. As a result of glycosylation, it migrates as an approximately 50-55 kDa band in SDS-PAGE under reducing conditions.