ACVR2B Protein (C-human IgG1-Fc & His, Mouse)

1. Measured by its ability to bind biotinylated Human INHBA-his (Cat:10429-H08H) in functional ELISA. 2. Measured by its ability to bind biotinylated mouse INHBA-his (Cat:50659-M08H) in functional ELISA. 3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 10-50 ng/mL in the presence of 10 ng/mL recombinant Activin A.

The recombinant mouse ACVR2B/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 364 amino acids and has a predicted molecular mass of 41 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmACVR2B/Fc monomer is approximately 60-65 kDa due to glycosylation.