ACVR2B Protein (C-human IgG1-Fc & His, Mouse)

Product Specifications

Abbreviation

ACVR2B

Synonyms

4930516B21Rik, ActRIIB, Acvr2b, AI047905

Host

HEK 293

Tag

C-human IgG1-Fc & His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1. Measured by its ability to bind biotinylated Human INHBA-his (Cat:10429-H08H) in functional ELISA. 2. Measured by its ability to bind biotinylated mouse INHBA-his (Cat:50659-M08H) in functional ELISA. 3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 10-50 ng/mL in the presence of 10 ng/mL recombinant Activin A.

Function

Met1-Thr134

Molecular Weight

The recombinant mouse ACVR2B/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 364 amino acids and has a predicted molecular mass of 41 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmACVR2B/Fc monomer is approximately 60-65 kDa due to glycosylation.