ACE2 Protein (C-His, Rhesus)

Product Specifications

Abbreviation

ACE2

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1.Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK (Dnp) -OH (Catalog # ES007) .The specific activity is >200 pmol/min/μg (Routinely tested) . 2.Immobilized Recombinant Rhesus ACE2 / Angiotensin-Converting Enzyme 2 Protein (His Tag) (Cat: 90211-C08H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Spike RBD-AVI&His Recombinant Protein, Biotinylated (Cat: 40592-V27B-B), the EC50 is 15-45 ng/mL (QC tested) . 3.Immobilized Recombinant Rhesus ACE2 / Angiotensin-Converting Enzyme 2 Protein (His Tag) (Cat: 90211-C08H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 (2019-nCoV) Spike S1-Fc Recombinant Protein (Cat: 40591-V02H), the EC50 is 12.5 ng/mL (Routinely tested) .