AA2AR Protein (N-Flag & C-His, Human)
Product Specifications
Abbreviation
AA2AR
Host
HEK 293
Tag
N-Flag & C-His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
≥ 95 % as determined by SDS-PAGE.
Bioactivity
1.Immobilized Recombinant Human ADORA2A-Nanodisc Protein (Cat: 12307-H18H-NA) at 5 μg/mL (100 μL/well) can bind AT004-Derived Anti-Human ADORA2A Monoclonal Antibody, Mouse IgG2a, the EC50 is 2-6 ng/mL. 2.Loaded Human A2aR -Nanodisc Protein, His & Flag Tag (Cat. No. 12307-H18H-NA) on AR2G Biosensor via Anti-Fusion Protein Monoclonal antibody, Mouse IgG2a, can bind AT004-Derived Anti-Human ADORA2A Monoclonal antibody, Mouse IgG2a with an affinity constant of 0.847nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Loaded AT004-Derived Anti-Human ADORA2A Monoclonal antibody, Mouse IgG2a on ProA Biosensor, can bind Human A2aR -Nanodisc Protein, His & Flag Tag (Cat. No. 12307-H18H-NA) with an affinity constant of 2.34 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .
Function
Pro2-Leu208 & Glu219-Ala316
Molecular Weight
The apparent molecular mass of the protein is approximately 41 kDa in SDS-PAGE under reducing conditions. FP6 predicts a molecular mass of 11.83 kDa
Species
Human
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