VEGFA Protein (Native, Human, Cynomolgus)

1.iPSC-derived human vascular organoids (Day 7) were cultured with FGF2 (Cat#10014-HNAE), VEGFA (Cat# 11066-HNAH), EGF (Cat#10605-HNAE) . Red arrows represent vascular organoids. Image taken at 10x magnification. (Routinely tested) 2. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) . The ED50 for this effect is typically 2-10 ng/mL. 3. Labeled biotin to VEGF165 Protein, Human / Cynomolgus, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of VEGF165 Protein, Human / Cynomolgus, Recombinant, Biotinylated (Cat:11066-HNAH-B) bound to Avastin was 0.1 nM. 4. Anti-VEGF (Research Grade Bevaciz*mab Biosimilar) captured on Protein A chip can bind Recombinant Human, Cynomolgus VEGF165 Protein (Cat. No. 11066-HNAH) with an affinity constant of 14.9 pM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 5.Loaded Biotinylated Recombinant Human VEGFR1/FLT1 Protein, His & AVI Tag (Cat. No. 10136-H49H-B) on SA Biosensor, can bind Recombinant VEGF165 Protein, Human, Cynomolgus (Cat. No. 11066-HNAH) with an affinity constant of 0.506 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human / cynomolgus VEGF165 consists of 165 amino acids and predicts a molecular mass of 19.2 KDa. It migrates as an approximately 22 kDa and 24 kDa band in SDS-PAGE under reducing conditions.