VEGFA Protein (Native, Human, Cynomolgus)

Product Specifications

Abbreviation

VEGFA

Host

Baculovirus-insect Cells

Tag

Native

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE.

Bioactivity

Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) .The ED50 for this effect is typically 1-10ng/mL.

Function

Met1-Arg191

Molecular Weight

The recombinant human / cynomolgus VEGF165 consists of 165 amino acids after removal of the signal peptide and predicts a molecular mass of 19.2 kDa. Due to different glycosylation, the apparent molecular mass of human VEGF165 is approximately 20 and 22 kDa in SDS-PAGE under reducing conditions, corresponding to the monomer.