TNFSF9 Protein (N-His, Mouse)

1.Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse 4-1BB Ligand at 20 μg/ml (100ul/well) can bind human 4-1BB with a linear range of 15.6-500 ng/ml 2.Loaded Recombinant Mouse 4-1BB/CD137 Protein, hFc Tag (Cat. No. 50811-M02H) on proA Biosensor, can bind Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein, His Tag (Cat. No. 50067-M07H) with an affinity constant of 0.807 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The secreted recombinant mouse TNFSF9 consists of 222 amino acids and has a calculated molecular mass of 25 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 40-45 kDa protein in SDS-PAGE under reducing conditions.