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TNFSF15 Protein (N-His, Cynomolgus, Rhesus)

Product Specifications

Abbreviation

TNFSF15

Host

HEK 293

Tag

N-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-MALS.

Bioactivity

1.Immobilized Recombinant Human DCR3 / TNFRSF6B Protein (Fc Tag) (Cat: 10224-H02B) at 2 μg/ml (100 μl/well) can bind Recombinant Cynomolgus / Rhesus TL1A / TNFSF15 Protein (His Tag) (Cat: 91015-C07H1), the EC50 is 8-25 ng/mL. 2.Loaded Recombinant Human DcR3/TNFRSF6B Protein, hFc Tag (Cat. No. 10224-H02B) on ProA Biosensor, can bind Recombinant Cynomolgus, Rhesus TL1A/TNFSF15 Protein, His Tag (Cat. No. 91015-C07H1) with an affinity constant of 4.02 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Anti-TNFSF15 (Research Grade Tulisoki*art Biosimilar) captured on Protein A chip can bind Recombinant Cynomolgus, Rhesus TL1A/TNFSF15 Protein , His Tag (Cat. No. 91015-C07H1) with an affinity constant of 0.138 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .

Function

Leu72-Leu251

Molecular Weight

The recombinant Cynomolgus/Rhesus TNFSF15 has a calculated molecular mass of 66.09 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 74.4 kDa protein in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 75.4 kDa verified by SEC-MALS .

Species

Cynomolgus, Rhesus

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