TNFRSF6B Protein (C-human IgG1-Fc, Human)

1.Measured by its ability to inhibit Fas Ligand induced apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 3-25 μg/mL in the presence of 200 ng/mL recombinant human Fas ligand. 2.Immobilized Recombinant Human DCR3 / TNFRSF6B Protein (Fc Tag) (Cat: 10224-H02B) at 2 μg/mL (100 μl/well) can bind Recombinant Human TL1A / TNFSF15 Protein (His Tag) (Cat: 17049-H07H), the EC50 is 60-180 ng/mL. 3.Loaded Recombinant Human DcR3/TNFRSF6B Protein, hFc Tag (Cat. No. 10224-H02B) on ProA Biosensor, can bind Recombinant Cynomolgus, Rhesus TL1A/TNFSF15 Protein, His Tag (Cat. No. 91015-C07H1) with an affinity constant of 4.02 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Recombinant Human DcR3/TNFRSF6B Protein , hFc Tag (Cat. No. 10224-H02B) captured on Protein A chip can bind Recombinant Human TL1A/TNFSF15 Protein (Trimer), His Tag (Cat. No. 17049-H07H2) with an affinity constant of 27.64 pM as determined in an SPR assay (Biacore T200) (Routinely tested) .

The recombinant human DCR3/Fc is a disulfide-linked homodimer. The reduced monomer comprises 508 amino acids and has a predicted molecular mass of 56.4 kDa. The apparent molecular mass of the protein is approximately 65 kDa in SDS-PAGE under reducing conditions.