TNFRSF4 Protein (C-His, Human)

1.Measured by its binding ability in a functional ELISA. Immobilized TNFRSF4-His (Cat: 10481-H08H) at 2 μg/ml (100 μl/well) can bind human TNFSF4-mFc (Cat: 13127-H04H), the EC50 of human TNFSF4-mFc is 60-240 ng/mL. 2.Loaded Recombinant Human OX40 Ligand/TNFSF4 Protein, hFc Tag (Cat. No. 13127-H01H) on ProA Biosensor, can bind Recombinant Human OX40/TNFRSF4 Protein, His Tag (Cat. No. 10481-H08H) with an affinity constant of 0.909 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Loaded Vonlerolizumab on ProA Biosensor, can bind Recombinant Human OX40/TNFRSF4 Protein, His Tag (Cat. No. 10481-H08H) with an affinity constant of 1.33 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Loaded Recombinant Human TNFRSF4 / OX40 / CD134 Protein (His Tag) (Cat. No. 10481-H08H) on Ni-NTA Biosensor, can bind Recombinant Human OX-40L / TNFSF4 / CD252 Protein (ECD, Fc Tag) (Cat. No. 13127-H04H) with an affinity constant of 10.31 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human TNFRSF4 consists of 199 amino acids after removal of the signal peptide and has a predicted molecular mass of 21.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TNFRSF4 is approximately 40-45 kDa due to glycosylation.