TNFRSF1B Protein (C-His, Human)

1.Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 30-180 ng/mL in the presence of 0.25 ng/mL recombinant human TNF-α (routinely tested) . 2.Immobilized Recombinant Human TNF-alpha / TNFA Protein (Cat: 10602-HNAE) at 2 μg/ml (100 μl/well) can bind Recombinant Human TNFR2/CD120b Protein (aa 1-268,196 Met/Arg, His Tag) (Cat: 10417-H08H1), the EC50 is 2.0-12.0 ng/mL.

The recombinant human TNFRSF1B comprises 257 amino acids and has a predicted molecular mass of 27.7 kDa. The apparent molecular mass of the protein is approximately 43 kDa in SDS-PAGE under reducing conditions due to glycosylation.