TNF Protein (Native, Mouse)
Product Specifications
Abbreviation
TNF
Synonyms
DIF, Tnf, TNF-a, TNF-alpha, Tnfa, TNFalpha, Tnfsf1a, TNFSF2, Tnlg1f
Host
E. coli
Tag
Native
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
≥ 95 % as determined by SDS-PAGE
Bioactivity
1.Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 1.5-15 pg/mL. 2.Immobilized Recombinant Mouse TNF-alpha / TNFA Protein (ECD) (Cat: 50349-MNAE) at 2 μg/mL (100 μL/well) can bind Anti-TNFa (Research Grade Adalim*mab Biosimilar), the EC50 is 0.9-2.7 ng/mL (Routinely tested) . 3.Immobilized Recombinant Mouse TNF-alpha / TNFA Protein (ECD) (Cat: 50349-MNAE) at 2 μg/mL (100 μL/well) can bind Recombinant Mouse TNFR1 / CD120a / TNFRSF1A Protein (Fc Tag) (Cat: 50496-M02H), the EC50 is 2-6 ng/mL (QC tested) .
Function
Leu80-Leu235
Molecular Weight
The recombinant mouse TNF-α consists of 157 amino acids and migrates with an apparent molecular mass of 17.39 kDa as estimated in SDS-PAGE under reducing conditions.
Species
Mouse
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