TIGIT Protein (C-His, Human)

1.Immobilized Recombinant Human CD155 / PVR / NECL5 Protein (Fc Tag) (Cat:10109-H02H) at 2μg/mL (100μL/well) can bind Recombinant Human TIGIT / VSTM3 Protein (His Tag) (Cat:10917-H08H3), the EC50 is 6-18 ng/mL (QC tested) . 2.Immobilized Anti-Tigit (Research Grade Clone Tiragolumab) at 2 μg/mL (100 μL/well) can bind Recombinant Human TIGIT / VSTM3 Protein (His Tag) (Cat: 10917-H08H3), the EC50 is 1.2-4 ng/mL (Routinely tested) . 3.Loaded Recombinant Human CD155/PVR Protein, hFc Tag (Cat. No. 10109-H02H) on ProA Biosensor, can bind Recombinant Human TIGIT Protein, His Tag (Cat. No. 10917-H08H3) with an affinity constant of 0.51 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Recombinant Human CD155/PVR Protein, hFc Tag (Cat. No. 10109-H02H) captured on Protein A chip can bind Recombinant Human TIGIT Protein (Dimer), His Tag (Cat. No. 10917-H08H3) with an affinity constant of 3.28 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 5.Anti-Tigit (Research Grade Clone MITG7192A) captured on Protein A chip, can bind Recombinant Human TIGIT / VSTM3 Protein (His Tag) (Cat: 10917-H08H3) with an affinity constant of 48.600 pM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 6.Immobilized Recombinant Human TIGIT / VSTM3 Protein (His Tag) (Cat: 10917-H08H3) at 1 μg/mL (100 μL/well) can bind Recombinant Human Nectin 3 Protein (Fc Tag) (Cat: 10852-H02H), the EC50 is 2.4 μg/mL (Routinely tested) . 7.Serial dilutions of anti-TIGIT (Research Grade Tiragolumab biosimilar) were added into Recombinant Human TIGIT / VSTM3 Protein (His Tag) (Cat: 10917-H08H3) : Recombinant Human CD155 / PVR Protein (ECD, Fc & AVI Tag), Biotinylated (Cat: 10109-H41H-B) binding reactions. The half maximal inhibitory concentration (IC50) is 1014.8 ng/mL (Routinely tested) .

The recombinant human TIGIT predicts a molecular mass of 28.81 kDa. It migrates as an approximately 36.72 kDa band in SDS-PAGE under reducing conditions.