TIGIT Protein (C-human IgG1-Fc, Human)

1.Loaded Recombinant Human TIGIT Protein, hFc Tag (Cat.No. 10917-H02H) on ProA Biosensor, can bind Recombinant Human CD155/PVR Protein, His Tag (Cat.No. 10109-H08H) with an affinity constant of 0.18 uM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 2.Immobilized Recombinant Human TIGIT / VSTM3 Protein (Fc Tag) (Cat: 10917-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD155 / PVR Protein (ECD, Fc & AVI Tag), Biotinylated (Cat: 10109-H41H-B), the EC50 is 5-15 ng/mL. (QC tested) 3.Immobilized Recombinant Human TIGIT / VSTM3 Protein (Fc Tag) (Cat: 10917-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human Nectin-2 Protein (Fc & AVI Tag), Biotinylated (Cat: 10005-H41H-B), the EC50 is 230-690 ng/mL. (Routinely tested) 4.Immobilized Recombinant Human CD155 / PVR Protein (His Tag) (Cat: 10109-H08H) at 2.5 μg/mL (100 μL/well) can bind Recombinant Human TIGIT / VSTM3 Protein (Fc Tag) (Cat: 10917-H02H), the EC50 is 60-180 ng/mL. (Routinely tested) 5.Immobilized Recombinant Human TIGIT / VSTM3 Protein (Fc Tag) (Cat: 10917-H02H) at 2 μg/mL (100 μL/well) can bind Anti-Tigit Antibody, Human IgG1 (Tiragolumab), the EC50 is 4-12 ng/mL (Routinely tested) .

The recombinant human TIGIT consists 353 amino acids and predicts a molecular mass of 39.2 kDa. As a result of glycosylation, human TIGIT migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.