TGFB1 Protein (C-His, Human)

Product Specifications

Abbreviation

TGFB1

Synonyms

CED, DPD1, IBDIMDE, LAP, TGF-beta1, TGFB, TGFbeta

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

1.Measured by its binding ability in a functional ELISA. Immobilized Human TGF beta 1 His (Cat: 10804-H08H) at 2 μg/ml (100 μl/well) can bind Human TGFBR2 His & hFc (Cat: 10358-H03H), the EC50 of Human TGFBR2 His & hFc is 5-35 ng/mL. 2.Loaded Recombinant Human TGF-beta RII/TGFBR2 Protein, hFc Tag (Cat. No. 10358-H02H) on ProA Biosensor, can bind Recombinant Human TGF beta 1 Protein (Latent), His Tag (Cat. No. 10804-H08H) with an affinity constant of 47.1 nM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested) .

Function

Met1-Ser390

Molecular Weight

The recombinant human latent TGFβ1 consists of 370 amino acids and has a calculated molecular mass of 42.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TGFβ1 is approximately 16, 38 and 55 kDa corresponding to mature TGFβ1, LAP protein and inacitve latent TGFβ1 respectively due to glycosylation. In non-reduced SDS-PAGE, it migrates as an approximately 110 kDa protein consisting of a TGFβ1 homodimer non-covalently linked to a LAP homodimer.