TFRC Protein (N-His, Human)
Product Specifications
Abbreviation
TFRC
Synonyms
CD71, IMD46, p90, T9, TFR, TFR1, TR, TRFR
Host
HEK 293
Tag
N-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .
Bioactivity
1.Immobilized Recombinant Human Transferrin Receptor / TFRC / CD71 Protein (ECD, His Tag) (Cat: 11020-H07H) at 2 μg/ml (100 μl/well) can bind Recombinant Human Transferrin Protein, Biotinylated (Cat: 11019-HNAH2-B), the EC50 is 4-15 ng/mL (QC tested) . 2.Recombinant Human Transferrin Receptor / TFRC / CD71 Protein (ECD, His Tag) (Cat: 11020-H07H) captured on CM5 chip via Anti-His antibody, can bind Recombinant Human Transferrin Protein (Cat: 11019-HNAH2) with an affinity constant of 63.500 nM as determined in an SPR assay (Biacore T200) (QC tested) .
Function
Cys89-Phe760
Molecular Weight
The recombinant human TFRC comprises 688 amino acids and has a predicted molecular mass of 77.37 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 74 kDa protein in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 160.3 kDa verified by SEC-MALS (Routinely tested) .
Species
Human
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