Spike Protein (C-His, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) (Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1 (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H) -His (Cat:40591-V08H12), the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1 (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H) -His (Cat:40591-V08H12) is 10-60 ng/mL.

Function

Met1-Arg685 (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H)

Molecular Weight

The recombinant SARS-CoV-2 (2019-nCoV) Spike S1 (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H) -His Recombinant Protein consists of 678 amino acids and predicts a molecular mass of 76.1 kDa. As a result of glycosylation, it migrates as an approximately 112.2 kDa band in SDS-PAGE under reducing conditions.