Spike Protein (C-mouse IgG1-Fc, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-mouse IgG1-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.

Bioactivity

Immobilized SARS-CoV-2 B.1.1.529 (Omicron) Spike RBD Protein (mFc Tag) (Cat:40592-V05H3) at 2 μg/mL (100 μL/well) can bind ACE2 Protein, Human, Recombinant (His Tag), HPLC-verified (Cat:10108-H08H), the EC50 is 30-150 ng/mL.

Function

Arg319-Phe541

Molecular Weight

The recombinant SARS-CoV-2 Spike RBD consists of 457 amino acids and predicts a molecular mass of 51.78 kDa. As a result of glycosylation, it migrates as an approximately 56.20 kDa band in SDS-PAGE under reducing conditions.