Spike Protein (C-mouse IgG1-Fc, SARS-CoV-2)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-mouse IgG1-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.

Bioactivity

1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag) (40592-V05H), the EC50 of SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag) is 10-30 ng/mL. 2. Loaded Human ACE2 Protein (His tag) (Cat. No. 10108-H08H) on SA Biosensor, can bind SARS-CoV-2/2019-nCoV Spike/RBD Protein (RBD, mFc Tag) (Cat. No. 40592-V05H) with an affinity constant of 0.562 nM as determined by Octet RED System. 3.Loaded Recombinant Human ACE2 Protein, His Tag, Biotinylated (Cat. No. 10108-H27B-B) on SA Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein (Cat. No. 40592-V05H) with an affinity constant of 1.75 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .