SFRP4 Protein (C-His, Human)

Product Specifications

Abbreviation

SFRP4

Synonyms

FRP-4, FRPHE, FRZB-2, PYL, sFRP-4

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T 1/2 2A6 mouse embryonal fibroblast cells. The ED50 for this effect is typically 1-6 μg/mL in the presence of 20 ng/mL of Wnt3a.

Function

Met1-Val346

Molecular Weight

The recombinant human sFRP4 consists of 336 amino acids after removal of the signal peptide and has a predicted molecular mass of 39 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 55-60 kDa due to glycosylation.