RSV-F Protein (C-His, RSV)
Product Specifications
Abbreviation
RSV-F
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-MALS.
Bioactivity
1.Immobilized Recombinant RSVB Post-fusion glycoprotein F/ RSV post-F Protein (His Tag) (Cat: 40999-V08H) at 1 μg/mL (100μL/well) can bind Anti-Fusion glycoprotein F0 Antibody, Human IgG1 (site II) (Palivizumab), the EC50 is 10-30 ng/mL and not bind Anti-Fusion glycoprotein F0 Antibody, Human IgG1 (Site Ø) (Nirsevimab) . 2.Immobilized Human respiratory syncytial virus (RSV) (A2) Prefusion glycoprotein F/RSV-preF Protein (Cat: 11049-VNAS) at 1 μg/mL (100μL/well) can bind Anti-Fusion glycoprotein F0 Antibody, Human IgG1 (Site Ø) (Nirsevimab), the EC50 is 6-18 ng/mL. Recombinant RSVB Post-fusion glycoprotein F/ RSV post-F Protein (His Tag) (Cat: 40999-V08H) is verified not recoginized by Anti-Fusion glycoprotein F0 Antibody, Human IgG1 (Site Ø) (Nirsevimab) .
Molecular Weight
The recombinant RSVB (BA9) Post-fusion glycoprotein F/ RSV post-F has a predicted molecular mass of 56.13 kDa.It migrates as an approximately 42.3 kDa and 21.1 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 170.8 kDa verified by SEC-MALS.
Species
RSV
Curated Selection
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