PVR Protein (C-human IgG1-Fc, Human)

1.Immobilized Recombinant Human TIGIT / VSTM3 Protein (His Tag) (Cat:10917-H08H3) at 2 μg/mL (100 μl/well) can bind Recombinant Human CD155/PVR/NECL5 Protein (Fc Tag) (Cat:10109-H02H), the EC50 is 10-30 ng/mL. 2.Loaded Recombinant Human CD155/PVR Protein, hFc Tag (Cat. No. 10109-H02H) on ProA Biosensor, can bind Recombinant Human TIGIT Protein, His Tag (Cat. No. 10917-H08H3) with an affinity constant of 0.51 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Loaded Recombinant Human CD155/PVR Protein, hFc Tag (Cat. No. 10109-H02H) on ProA Biosensor, can bind Recombinant Human DNAM-1/CD226 Protein, His Tag (Cat. No. 10565-H08H) with an affinity constant of 0.386 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Recombinant Human CD155/PVR Protein, hFc Tag (Cat. No. 10109-H02H) captured on Protein A chip can bind Recombinant Human TIGIT Protein (Dimer), His Tag (Cat. No. 10917-H08H3) with an affinity constant of 3.28 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

The recombinant human CD155/Fc is a disulfide-linked homodimer. The reduced monomer consists of 561 amino acids and predicts a molecular mass of 61.8 kDa. As a result of glycosylation, the rhCD155/Fc monomer migrates as approximately 95-105 kDa band in SDS-PAGE under reducing conditions.