PLAU Protein (C-His, Human)

Product Specifications

Abbreviation

PLAU

Synonyms

ATF, BDPLT5, QPD, u-PA, UPA, URK

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 97 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC) . The specific activity is >2000 pmoles/min/μg.

Function

Met1-Leu431

Molecular Weight

The secreted recombinant human PLAU comprises 422 amino acids with a predicted molecular mass of 47.8 kDa. As a result of glycosylation and cleavage, rhPLAU migrates as three bands corresponding to the long α chain, β chain and unprocessed full-length PLAU with the molecular mass of 19.1, 35.4 and 51 kDa respectively in SDS-PAGE under non-reducing conditions.