PDCD1LG2 Protein (C-His, Mouse)

Product Specifications

Abbreviation

PDCD1LG2

Synonyms

B7-DC, B7dc, Btdc, Cd273, F730015O22Rik, PD-L2, Pdcd1lg2, Pdl2

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse PD-L2-his at 10 μg/mL (100 μl/well) can bind mouse PD1-Fc. The EC50 of mouse PD1-Fc is 1.63 μg/mL. 3.Loaded Recombinant Mouse PD-1 Protein, hFc Tag (Cat. No. 50124-M02H) on proA Biosensor, can bind Recombinant Mouse PD-L2/B7-DC Protein, His Tag (Cat. No. 50804-M08H) with an affinity constant of 80.700 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-Arg219

Molecular Weight

The secreted recombinant mouse PDCD1LG2 comprises 211 amino acids and has a calculated molecular mass of 24 kDa. As a result of glycosylation, the apparent molecular mass of rmPDCD1LG2 is approximately 35-45 kDa in SDS-PAGE under reducing conditions.