PDCD1 Protein (C-human IgG1-Fc, Human)

1.Immobilized PD-1 Protein, Human, Recombinant (ECD, hFc Tag) (Cat:10377-H02H) at 2 μg/mL (100 μL/well) can bind PD-L2 Protein, Human, Recombinant (ECD, His Tag) (Cat:10292-H08H), the EC50 is 45-280ng/mL (QC tested) . 2.Immobilized Recombinant Human PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat: 10377-H02H) at 2 μg/mL (100 μl/well) can bind Recombinant Human PD-L1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10084-H41H-B), the EC50 is 25-75 ng/mL (Routinely tested) . 3.Loaded Recombinant Human PD-1 Protein, hFc Tag (Cat. No. 10377-H02H) on ProA Biosensor, can bind Recombinant Human PD-L2 / B7-DC Protein, His Tag (Cat. No. 10292-H08H) with an affinity constant of 0.154 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Recombinant Human PD-1 Protein, hFc Tag (Cat. No. 10377-H02H) captured on Protein A chip, can bind Recombinant Human PD-L1/B7-H1 Protein, His Tag (Cat. No. 10084-H08H) with an affinity constant of 1.620 μM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 5.Recombinant Human B7-DC / PD-L2 / CD273 Protein (His Tag) (Cat: 10292-H08H) captured on CM5 chip via Anti-His antibody, can bind Recombinant Human PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat: 10377-H02H) with an affinity constant of 20.000 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 6.Loaded Recombinant Human PD-1 Protein (ECD, hFc Tag) (Cat. No. 10377-H02H) on proA Biosensor, can bind Recombinant Human PD-L1/B7-H1 Protein (ECD, His Tag) (Cat. No. 10084-H08H) with an affinity constant of 4.170 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 7.Immobilized Recombinant Human PD-L1 Protein (His & AVI Tag), Biotinylated (Cat: 10084-H49H-B) at 1 μg/mL (100 μl/well) on streptavidin precoated (5 μg/mL, 100 μL/well) can bind Recombinant Human PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat: 10377-H02H), the EC50 is 4.2 ng/mL (Routinely tested) . 8.Immobilized Recombinant Human PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat: 10377-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human PD-L2 Protein (Fc & AVI Tag), Biotinylated (Cat: 10292-H41H-B), the EC50 is 2.0 ng/mL (Routinely tested) . 9.Immobilized Recombinant Human B7-DC/PD-L2/CD273 Protein (His&AVI Tag), Biotinylated (Cat: 10292-H27H-B) at 1 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat: 10377-H02H), the EC50 is 1.7 ng/mL (Routinely tested) . 10.Serial dilutions of Anti-PD1 (Research Grade Nivolu*mab Biosimilar) were added into Recombinant Human PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat: 10377-H02H) : Recombinant Human PD-L1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10084-H41H-B) binding reactions. The half maximal inhibitory concentration (IC50) is 0.8-2.5 μg/mL (Routinely tested) .

The recombinant human PDCD1 consists of 381 amino acids and predicts a molecular mass of 42.7 kDa. As a result of glycosylation, human PDCD1 migrates as an approximately 55-66 kDa protein in SDS-PAGE under reducing conditions.